Journal: PLOS One

Article Title: Impaired dynein function preserves spinal interneuron survival and positioning in an ALS-like mouse model

doi: 10.1371/journal.pone.0346246

Figure Lengend Snippet: Number of ChAT + (A-C), GAD-67 + (D), Calbindin + (E), Parvalbumin + (F), Parvalbumin + −Calbindin + (G), Parvalbumin + −GAD-67 + (H), and ChAT + −GAD-67 + (I) cells per biological replicate. The number of cells analysed is the sum of immunostained positive cells from eight spinal cord cross-sections (two sections per lumbar level L3–L6) per biological replicate. Data are shown as mean ± standard deviation and were analysed by an ordinary one-way ANOVA. N = 3 biological replicates per genotype.

Article Snippet: Following three 10-min PBS washes at 100 rpm, secondary probing was performed for 3 hours in the dark in PBS containing 0.05% Tween-20 and the relevant secondary antibodies for ChAT (1:250 dilution, donkey anti-goat IgG H + L Alexa Fluor ® 405, Abcam, ab175664), Calbindin (1:500 dilution, Rhodamine TRITC-AffiniPure donkey anti-guinea pig Ig G H + L, Jackson Immuno Research, 706-025-148-JIR), Parvalbumin (1:500 dilution, Alexa Fluor 647-AffiniPure donkey anti-rabbit IgG H + L, Jackson Immuno Research, 711-605-152), and GAD-67 (1:500 dilution, donkey anti-chicken IgY H + L Highly Cross Adsorbed Alexa Fluor ® 488, Life Technologies, A78948).

Techniques: Standard Deviation

Journal: Frontiers in Molecular Neuroscience

Article Title: Connexin30.2: In Vitro Interaction with Connexin36 in HeLa Cells and Expression in AII Amacrine Cells and Intrinsically Photosensitive Ganglion Cells in the Mouse Retina

doi: 10.3389/fnmol.2016.00036

Figure Lengend Snippet: List of primary antibodies used in this study .

Article Snippet: Calretinin , Guinea pig calretinin, full length amino acid sequence , Goat, polyclonal , 1:500 , Merck-Millipore, Darmstadt, Germany (AB 1550).

Techniques: Recombinant, Sequencing, Derivative Assay, Purification

Journal: Frontiers in Molecular Neuroscience

Article Title: Connexin30.2: In Vitro Interaction with Connexin36 in HeLa Cells and Expression in AII Amacrine Cells and Intrinsically Photosensitive Ganglion Cells in the Mouse Retina

doi: 10.3389/fnmol.2016.00036

Figure Lengend Snippet: Colocalization (coloc.) of β-galactosidase immunoreactivity with different calcium-binding proteins (CaBP) .

Article Snippet: Calretinin , Guinea pig calretinin, full length amino acid sequence , Goat, polyclonal , 1:500 , Merck-Millipore, Darmstadt, Germany (AB 1550).

Techniques:

Journal: Frontiers in Molecular Neuroscience

Article Title: Connexin30.2: In Vitro Interaction with Connexin36 in HeLa Cells and Expression in AII Amacrine Cells and Intrinsically Photosensitive Ganglion Cells in the Mouse Retina

doi: 10.3389/fnmol.2016.00036

Figure Lengend Snippet: Cx30.2 lacZ -expressing neurons belong to several different populations of amacrine and ganglion cells, including intrinsically photosensitive retinal ganglion cells (ipRGCs). (A–H) Double immunofluorescence stainings for β-gal (A 1 –H 1 ) and calbindin (A 2 ,B 2 ) , calretinin (C 2 ,D 2 ) , parvalbumin (E 2 ,F 2 ) , and melanopsin (G 2 ,H 2 ) in the INL (A/C/E/G) and GCL (B,D,F,H) of Cx30.2 lacZ/lacZ whole-mount retinae. (A 3 –H 3 ) show overlays of the respective single channels. Arrows indicate example cells that express both antigens, double arrows indicate cells that express β-gal but lack the respective marker, and arrowheads indicate cells expressing the marker but lacking β-gal. Insets in (G 1 ) show intensity-enhanced magnifications of the areas marked by dashed squares to verify colocalization with melanopsin. Scale bar: 50 μm.

Article Snippet: Calretinin , Guinea pig calretinin, full length amino acid sequence , Goat, polyclonal , 1:500 , Merck-Millipore, Darmstadt, Germany (AB 1550).

Techniques: Expressing, Immunofluorescence, Marker